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Technical Data Sheet

WP2101

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WASP / N-WASP
Rabbit Polyclonal
Price
Size
Species Reactivity
MW

$295
100 μl
Hu, Rt, Ms
63 kDa

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Western blot analysis of Jurkat (lane 1), A431 (lane 2), and HeLa (lane 3) cell lysates (20 µg/lane). Blots were probed with rabbit polyclonal anti-WASP/N-WASP (WP2101).

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Immunocytochemical labeling of N-WASP in control and pervanadate-treated A431 cells. The cells were labeled with rabbit polyclonal N-WASP/WASP (WP2101) or rabbit polyclonal N-WASP (Tyr-256) antibodies, then the antibodies were detected using appropriate secondary antibody conjugated to DyLight® 594.

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Application
Dilution


ELISA
1:2000


ICC
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
Product References
Cheney, L. et al. (2011) J Infectious Diseases. 203:1824. WB: mouse adipocytes
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Background
Members of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Serine and tyrosine phosphorylation regulates the activity of both proteins. WASP is observed as a 63 kDa protein in hematopoietic cells, while N-WASP is observed as a 65 kDa in many tissues, especially brain.


Background References
Baba, Y. et al. (1999) Blood 93:2003.
Higgs, H.N. & Pollard, T.D. (2001) Annu Rev Biochem 70:649-676.
Cory, G.O. et al. (2003) Mol Cell. 11(5):1229-39.
Immunogen
A synthetic peptide (coupled to KLH) corresponding to a region near the C-terminus of human WASP. This sequence is highly homologous with a similar region in human N-WASP.
Buffer and Storage
Rabbit polyclonal, affinity-purified antibody is supplied in 100µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
This antibody detects a 63 kDa* protein corresponding to the molecular mass of WASP on SDS-PAGE immunoblots of Jurkat cell lysate. In rat brain, A431, and SKN-SH cells, this antibody detects a 65 kDa* protein corresponding to the molecular mass of N-WASP.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
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N-WASP Rabbit Polyclonal

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WP2401
unphosphorylated N-WASP (Ser-484/Ser-485) Rabbit Polyclonal

WP2601
N-WASP (Tyr-256), phospho-specific Rabbit Polyclonal

WX2005
N-WASP Blocking Peptide

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