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Technical Data Sheet

PL7141

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PC12 (NGF-differentiated)
Lysate
Price
Size

$75
100 μl

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PC12 cells grown on rat tail collagen were undifferentiated (Control) or differentiated (200 ng/ml NGF) for 48 hrs. The cells were then fixed and labeled for immunocytochemistry using anti-βIII-Tubulin (C-terminus; TP1691).

Application
Dilution


WB
20 μl/lane



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
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Background
Rat pheochromocytoma-derived PC12 cells are an important cell model for studies of growth factor cell signaling pathways. PC12 has been used for a variety of studies where cell division and cell differentaiton have been induced through growth factor activation. Both NGF and FGF can promote neurite proliferation, while EGF and IGF-1 can induce mitosis in PC12 cells. In addition, PC12 cells can be used as a model of neuron development since they differentiate into a neuron-like morphology and express various neuronal proteins.

PC12 cells were differentiated into neurite-bearing cells by plating the cells on rat tail collagen coated dishes and growing the cells in the presence of NGF (200 ng/ml) for 48 hrs (Cat.# PL7141). Undifferentiated PC12 cells (PL7121) were grown in suspension cultures. Both PC12 cell models were lysed in 1% SDS, 1.0 mM sodium ortho-vanadate, 1 mM sodium fluoride, 10 mM Tris (pH 7.4) buffer. Protein concentration was determined using the BCA method (Pierce) before diluting to final concentration and buffer.

Buffer and Storage
Cell Lysates are supplied at a concentration of 1 mg/ml in electrophoresis sample buffer (62.5 mM Tris pH 6.8, 2% SDS, 5% glycerol, 0.003% bromophenol blue, 0.9% β-mercaptoethanol). Store at –20°C. Do not boil or dilute. Stable for 1 year.
imageRelated Products
PL7091
PC12 Control Lysate

PL7101
PC12 + Calyculin A Lysate

PL7111
PC12 + Pervanadate Lysate

PL7121
PC12 (undifferentiated) Lysate

PL7131
PC12 + NGF (30 min) Lysate

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