ECM Logo Bar
Search :
Technical Data Sheet

MM0271

image
image
image
Mitofilin
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$295
100 μl
Hu
90 kDa

image
image
Enlarge

Western blot analysis of human cell lysates: MeWo (lane 1), MDA-MB-231 (lane 2), PC3 (lane 3), and A549 (lane 4). The blot was probed with mouse monoclonal anti-mitofilin (MM0271) at 1:1000.

image
Enlarge

Immunocytochemical labeling of mitofilin in methanol-acetone (1:1) fixed human MeWo cells. The cells were labeled with mouse monoclonal anti-mitofilin (clone M027). The antibody was detected using goat anti-mouse DyLight® 594.

image
Application
Dilution


ELISA
1:2000


ICC
1:100


IP
1:100


WB
1:1000



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
Mitofilin is an important organizer of mitochondrial architecture. The mitofilin sequence encodes a polypeptide with a central alpha-helical region with predicted coiled coil domains flanked by globular amino and carboxy termini. There are four isoforms of mitofilin, and 90/91 kDa mitofilin forms have been observed in western blots. Mitofilin is located in the inner mitochondrial membrane and interacts with several protein complexes of the outer membrane, thereby generating contact sites between the two membrane systems of mitochondria. These mitofilin-containing hetero-oligomeric protein complexes form the mitochondrial inner membrane organizing system (MINOS). MINOS integrity is required for the structural maintenance of the inner mitochondrial membrane. This mitochondrial region contains cristae membranes that form large tubular invaginations that protrude into the mitochondrial matrix. These cristae membranes contain the enzyme complexes of the oxidative phosphorylation machinery. MINOS deficiency causes loss of crista junction structures and the detachment of cristae from the inner boundary membrane.


Background References
Odgren PR, et al. (1996) J Cell Sci. 109(9):2253.
Zerbes RM, et al. (2012) Biol Chem. 393(11):1247.
Immunogen
Clone M027 was generated from a proprietary antigen related to human mitofilin expressed in the A431 epidermoid carcinoma cell line.
Buffer and Storage
Mouse monoclonal antibody purified with protein G chromatography is supplied in 100 µl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store at –20°C. Stable for 1 year.
Specificity
Clone M027 detects a 90 kDa* band corresponding to the molecular mass of mitofilin on SDS-PAGE immunoblots of native or denatured human PC3, MDA-MB-231, A431, A549, and MeWo cell lysates, as well as human breast, skin, and brain tissues. In addition, mass spectrometry analysis of immunoprecipitates using MM0271 in human A431 cell lysates confirms this antibody only detects Mitofilin protein. The antibody can be used for multiple applications including ELISA, western blot, immunocytochemistry, and immunoprecipitation.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
CK7700
Cell Structure Labeling Immunocytochemistry Kit

CK7720
Cytoskeletal Filament Labeling Immunocytochemistry Kit

GM3421
GM130 (C-terminal region) Mouse Monoclonal

HM4381
Hsp60 (N-terminal region) Mouse Monoclonal

SM3591
Smac/DIABLO (C-terminal region) Mouse Monoclonal

New Releases
NM0161
image
Na+/K+ ATPase β3 (Extracellular)
Mouse Monoclonal
image
IM0321
image
Integrin β1 (Extracellular region)
Mouse Monoclonal
image
FM0311
image
FXYD5/Dysadherin (Extracellular region)
Mouse Monoclonal
image