ECM Logo Bar
Search :
Technical Data Sheet

CM4711

image
image
image
CREB (a.a. 123-136)
Mouse Monoclonal IgG1
Price
Size
Species Reactivity
MW

$245
100 μl
Hu, Rt, Ms, Ck, F
43 kDa

image
image
Enlarge

Western blot analysis of CREB expression in HeLa cells. The blot was probed with mouse monoclonal CREB (a.a. 123-136) antibody (CM4711) at 1:250 dilution.

Application
Dilution


ELISA
1:1000


WB
1:500



End user should determine optimal dilution for their particular applications and experiments.Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature.
image
Background
CREB (cyclic AMP response element-binding protein) is a stimulus-induced transcription factor that plays pivotal roles in cell survival and proliferation. CREB is expressed in various tissues, and has important gene-regulating roles in the nervous system. The transactivation function of CREB is primarily regulated through Ser-133 phosphorylation by cAMP-dependent protein kinase A (PKA) and related kinases. CREB is phosphorylated at other sites in response to calcium influx and DNA damage. The DNA-damage responsive nuclear kinase, HIPK2, can phosphorylate Ser-271 but not Ser-133 in CREB, and this phosphorylation activates CREB transactivation function. Mutation of Ser-271 to Glu-271 potentiates the CREB transactivation function. Thus, phosphorylation of Ser-271 may be the mode of activation for CREB-dependent transcription in response to genotoxic stress.


Background References
Gouveia, S.M. & Akhmanova, A. (2010) Int Rev Cell Mol Biol. 285:1-74.
Sakamoto, K. et al. (2010) Mol. Biol. Cell 21:2966.
Lui, C. et al. (2012) IUBMB Life. 64(3):209.
Immunogen
Clone 82B514.2 was generated from a synthetic peptide (coupled to carrier protein) corresponding to amino acids 123 to 136 in human CREB. This sequence is well conserved in rat, mouse, chicken, and zebrafish CREB.
Buffer and Storage
Mouse monoclonal purified with protein G chromatography is supplied in 100μl phosphate-buffered saline with 0.2% gelatin and 0.05% sodium azide. Store at 4°C, stable for 6 months. For long term storage, aliquot and store at -20°C.
Specificity
The antibody detects a 43 kDa* protein corresponding to the molecular mass of CREB on SDS-PAGE immunoblots of human HeLa cells.

*All molecular weights (MW) are confirmed by comparison to Bio-Rad Rainbow Markers and to western blot mobilities of known proteins with similar MW.
imageRelated Products
CK6460
CREB Phospho-Regulation Antibody Sampler Kit

CM4151
CREB (Ser-133), phospho-specific Mouse Monoclonal

CP4141
CREB (N-terminal region) Rabbit Polyclonal

CP4161
CREB (Ser-271), phospho-specific Rabbit Polyclonal

EK6160
EGFR Phospho-Regulation Antibody Sampler Kit

MK6050
MAP Kinase Activation Antibody Sampler Kit

New Releases
CM0191
image
Cyclophilin B
Mouse Monoclonal
image
CK6740
image
VE-Cadherin Phospho-Regulation
Antibody Sampler Kit
image
AM0171
image
Annexin A5
Mouse Monoclonal
image