ECM Logo Bar
Search :
Technical Data Sheet

PK6580

image
image
image
Plexin A1, B1, and D1 Receptors
Antibody Sampler Kit
Price

$395

Kit Components
Applications: WB = Western blot, E = ELISA, ICC = Immunocytochemistry, IP = Immunoprecipitation, IHC = Immunohistochemistry Species: H = Human, R = Rat, M = Mouse, C = Chicken, F = Fish

Product References
PP1301 Cheadle, L. et al. (2014) J of Neuroscience 34(23): 7999. WB: rat brain
PP1841 Damola, A. et al. (2013) Prostate 73(12):1326. WB, IP: Prostate Cells, shRNA knockdown
PP1841 Soong, J. & Scott, G. (2013) J Cell Sci. 126(Pt 2):688. WB: human melanocytes
PP1841 Soong, J. et al (2012) J Invest Dermatol. 132(4):1230. WB: melanocyte, keratinocyte, smooth muscle
Kit Summary
The Plexin Receptor kit can be used to monitor the expression of Plexin A1, Plexin B1, and Plexin D1. The kit includes three antibodies for detecting different regions of Plexin D1, two antibodies to different regions in the Sema domain of Plexin A1, and an antibody that detects the C-terminal region of Plexin B1.
image
image
Enlarge

Western blot analysis of Plexin D1 expression in human endothelial cells (HUVEC) (lanes 1-6). The blots were probed with rabbit polyclonals anti-Plexin D1 (Sema domain) (lanes 1 & 2), anti-Plexin D1 (Cytoplasmic domain) (lanes 3 & 4), and anti-Plexin D1 (a.a. 1635-1647) (lanes 5 & 6). Each antibody was used in the presence of their respective blocking peptide (lanes 2, 4 & 6).

image
Enlarge

Western blots showing Cos7 cells transfected with Myc-tagged Plexin A1 (lanes 1 & 3), neonatal rat brain (lane 2), or Plexin-A1 immunoprecipitated from transfected cells using anti-Myc (lane 4) or anti-Plexin-A1 (PP1301; lane 5). These blots were probed with anti-Plexin-A1 (PP1301; lanes 1 & 2) or with anti-Myc (lanes 3-5).

image
Background
Plexins are a family of large integral membrane proteins that complex with neuropilins to form semaphorin co-receptors. Plexin A1 and its co-receptor Neuropilin 1 bind Sema-3A leading to collapse of neuronal axon growth cones. The expression of Plexin-A1 and its co-receptor is upregulated in neurons after central nervous system injury, and the Sema-3A activation of Plexin-A1 and Neuropilin-1 co-receptors may have significant roles in suppressing axon regeneration after neuronal injury. Plexin-B1 is expressed as a 300 kDa precursor protein that can be cleaved into a 200 kDa a-subunit and a 100 kDa b-subunit. This plexin is the receptor for the class IV semaphorin, Sema-4D, and the Sema-4D/Plexin B1 pathway may be involved in cell survival, cell migration, and angiogenesis. Plexin D1 is a type I transmembrane protein that may be expressed as multiple isoforms in many cell types, including neurons and endothelial cells. Semaphorin 3E (Sema-3E) and semaphorin 4A can bind Plexin D1 leading to phosphorylation of Tyr-1642 and activation of Plexin D1. The Sema3E/Plexin D1 pathway may have important roles in angiogenesis and metastasis.

Background References
Kolodkin, A.L. et al. (1993) Cell 75:1389.
Tamagnone, L. et al. (1999) Cell 99:71.
Takahashi, T. & Strittmatter, S.M. (2001) Neuron 29:429.
van der Zwaag, B. et al. (2002) Dev Dyn. 2002 Nov;225(3):336-43.
Artigiani, S. et al. (2003) J Biol. Chem. 278(12):10094.
Deutsch, U. (2004) Dev Cell. 7(1):1.
Basile, J.R. et al. (2006) PNAS 103(24):9017.
Casazza, A. et al. (2010) J Clin Invest. 120(8):2684.
Buffer and Storage
Rabbit polyclonal antibodies are supplied in phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide. Store all at –20°C. Stable for 1 year.
imageRelated Products
NP2111
Neuropilin-1 (a1 CUB Domain) Rabbit Polyclonal

PX1305
Plexin A1 (Sema Domain) Blocking Peptide

SM1881
Semaphorin-4D (C-terminal region) Mouse Monoclonal

SP1221
Semaphorin-3A (Central region) Rabbit Polyclonal

SP1241
Semaphorin-3A (C-terminal) Rabbit Polyclonal

SP1401
Semaphorin-3A (N-terminal) Rabbit Polyclonal

PX1475
Plexin A1 (Sema Domain) Blocking Peptide

PX1845
Plexin B1 (C-terminal region) Blocking Peptide

SK6570
Sema-3E and Plexin D1 Receptor Antibody Sampler Kit

SP4461
Semaphorin-3E (N-terminal region) Rabbit Polyclonal

New Releases
OM5741
image
Osteopontin (N-terminal region)
Mouse Monoclonal
image
CM5961
image
P-Cadherin (N-terminal region)
Mouse Monoclonal
image
GM0121
image
Girdin (C-terminus)
Mouse Monoclonal
image