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ECM Biosciences offers kits that pair our site-specific and phospho-specific, polyclonal and monoclonal antibodies with DyLightŪ conjujgated secondary reagents for use in immunocytochemsitry and immunohistochemistry applications.

 
Catalog #
Antibody Description
Host
Applications
Reactivity
Datasheet
AK7600 Actin Filament Regulation Immunocytochemistry Kit mAbs & pAbs ICC,IHC H,R,M,C
The actin filament regualtion kit can be used to compare labeling between actin filaments and total actin. The kit contains two antibodies for detecting total actin, and Phalloidin:FITC for labeling actin filaments. In addition, secondary reagents conjugated to DyLightŪ 594 are included for dual labeling experiments with Phalloidin:FITC.
 
AK7710 Actin Phospho-Regulation Immunocytochemistry Kit mAbs & pAbs ICC,IHC H,R,M,C
The actin phospho-regualtion kit can be used to label actin phosphorylation at Tyr-53 relative to total actin expression pattern. The kit contains anti-Actin (Tyr-53) phospho-specific antibody, and two antibodies for detecting total actin. In addition, secondary reagents conjugated to DyLightŪ 488 or DyLightŪ 594 are included for labeling primary antibodies for detection using green (493Ex/518Em) or red (593Ex/618Em) filter sets.
 
CK7610 β-Catenin Tyr-142 & Tyr-654 Phosphorylation Immunocytochemistry Kit mAbs & pAbs ICC,IHC H,R,M,C
The β-Catenin phospho-regulation kit can be used for immunocytochemical co-localization of β-Catenin phosphorylation at Tyr-142 and Tyr-654 relative to total β-Catenin. The kit includes rabbit polyconal and mouse monoclonal antibodies, along with goat-anti-rabbit conjugated to DyLightŪ 594 and goat anti-mouse conjugated to DyLightŪ 488 for dual labeling experiments.
 
CK7680 γ-Catenin Phospho-Regulation   Immunocytochemistry Kit mAbs & pAbs ICC,IHC H,R,M
The γ-Catenin phospho-regulation kit can be used for immunocytochemical co-localization of γ-Catenin phosphorylation at Tyr-550 compared to the total expression of γ-Catenin. The kit includes rabbit polyconal and mouse monoclonal antibodies, along with goat-anti-rabbit conjugated to DyLightŪ 594 and goat anti-mouse conjugated to DyLightŪ 488 for dual labeling experiments.
 
CK7650 δ1-Catenin Phospho-Regulation   Immunocytochemistry Kit mAbs ICC,IHC H,R,M
The δ1-Catenin phospho-regulation kit can be used for immunocytochemical labeling of phosphorylation at Tyr-228, Tyr-280, and Tyr-302. The kit includes a monoclonal antibody to examine the pattern of expression of the total population of δ1-Catenin. In addition, secondary reagents conjugated to DyLightŪ 488 or DyLightŪ 594 are included for labeling primary antibodies for detection using green (493Ex/518Em) or red (593Ex/618Em) filter sets.
 
CK7700 Cell Structure Labeling Immunocytochemistry Kit mAbs ICC,IHC H,R,M

The cell structure labeling kit can be used to identify important cell structures found in eukaryotic cells. The kit includes a panel of antibodies that detect the following cell structures:

Actin Filaments = Phalloidin:FITC    Cell Junctions = β-catenin

Focal Adhesions = Paxillin    Intermediate Filaments = Vimentin

Microtubules = α-Tubulin    Nuclear Envelope = Nucleoporin p62

 
CK7720 Cytoskeletal Filament Labeling Immunocytochemistry Kit mAbs ICC,IHC H,R,M
The cytoskeletal filament kit can be used to label the major cytoskeletal elements in cells: actin filaments (Phalloidin:FITC), intermediate filaments (Vimentin), and microtubules (α-tubulin). The kit includes Phalloidin:FITC and Goat anti-mouse conjugated to DyLightŪ 594 for for dual labeling experiments.
 
EK7620 ERK1/2 Phospho-Regulation Immunocytochemistry Kit mAbs ICC,IHC H,R,M,C,F
The ERK1/2 phospho-regulation kit may be used to examine the pattern of phosphorylation of ERK1 at the dual site (Thr-202/Tyr-204) relative to the total ERK1 pattern. The kit includes monoclonal antibodies to ERK1 (C-terminal region) and ERK1/2 (Thr-202/Tyr-204), as well as Goat anti-mouse secondary reagents conjugated to DyLightŪ 488 or DyLightŪ 594 for detection using green (493Ex/518Em) or red (593Ex/618Em) filter sets.
 
JK7630 JNK Phospho-Regulation Immunocytochemistry Kit mAbs ICC,IHC H,R,M,C,F
The JNK1 phospho-regulation kit may be used to examine the pattern of phosphorylation of JNK1 at the dual site (Thr-183/Tyr-185) relative to the total JNK1 pattern. The kit includes monoclonal antibodies and Goat anti-mouse secondary reagents conjugated to DyLightŪ 488 or DyLightŪ 594 for detection using green (493Ex/518Em) or red (593Ex/618Em) filter sets.
 
MK7640 Microtubule Labeling Immunocytochemistry Kit mAbs & pAbs ICC,IHC H,R,M
The microtubule lableing kit can be used for immunocytochemical labeling of tubulin. The kit includes monoclonal antibodies to α- and βI-tubulin, and a rabbit polyclonal antibody to bIII-tubulin. The kit also includes goat-anti-rabbit conjugated to DyLightŪ 594 and goat anti-mouse conjugated to DyLightŪ 488 for dual labeling experiments.
 
MK7730 Myosin Light Chain Phospho-Regulation Immunocytochemistry Kit mAbs & pAbs ICC,IHC H,R,M
The Myosin Regulatory Light Chain phospho-regulation kit can be used for immunocytochemical co-localization of MLC phosphorylation at Ser-1 and Ser-19 compared to the total expression of MLC. The kit includes rabbit polyconal and mouse monoclonal antibodies along with Goat-anti-rabbit conjugated to DyLightŪ 594 and Goat anti-mouse conjugated to DyLightŪ 488 for dual labeling experiments.
 
OK7670 Organelle Marker Immunocytochemistry Kit mAbs & pAbs ICC,IHC H,R,M

The organelle market kit can be used to co-localize proteins to specific organelles in cells. The kit includes a panel of antibodies that detect the following cell organelles:

Caveolae = Caveolin 1    Endoplasmic Reticulum = Calnexin

Endosomes = EEA1    Golgi = GM130

Mitochondria = Hsp60    Nucleus = Histone H2B

 
PK7690 p38 MAPK Phospho-Regulation Immunocytochemistry Kit mAbs & pAbs ICC,IHC H,R,M
The p38 MAPK phospho-regulation kit can be used for immunocytochemical co-localization of p38 MAPK phosphorylation at Thr-180/Tyr-182 and Tyr-323 relative to the total expression of p38 MAPK. The kit includes rabbit polyconal and mouse monoclonal antibodies along with Goat-anti-Rabbit conjugated to DyLightŪ 594 and Goat anti-Mouse conjugated to DyLightŪ 488 for dual labeling experiments.
 
PK7660 Paxillin Phospho-Regulation Immunocytochemistry Kit mAbs & pAbs ICC,IHC H,R,M
The paxillin phospho-regulation kit can be used for immunocytochemical co-localization of paxillin phosphorylation at Ser-83 compared to the total expression of paxillin. The kit includes rabbit polyconal and mouse monoclonal antibodies along with Goat-anti-rabbit conjugated to DyLightŪ 594 and Goat anti-mouse conjugated to DyLightŪ 488 for dual labeling experiments.
 
PK7740 Phospho-Serine, Threonine & Tyrosine Immunocytochemistry Kit mAbs & pAbs ICC,IHC H,R,M,C,F
The phosphoserine-threonine and phosphotyrosine regulation kit can be used to examine the pattern of phoshoserine-threonine relative to phoshotyrosine, or relative to a target protein. The kit includes monoclonal and polyclonal antibodies to phosphoserine-threonine and phosphotyrosine along with Goat-anti-Rabbit conjugated to DyLightŪ 488 and Goat anti-Mouse conjugated to DyLightŪ 594 for dual labeling experiments.
 
SK7750 Sema-3A & NRP1/Plexin A1 Receptor Immunocytochemistry Kit pAbs ICC,IHC H,R,M
The Sema-3A & NRP1/Plexin A1 receptor kit can be used for immuncytochemical labeling of Sema-3A relative to its receptors, NRP1 and Plexin A1. The kit also includes secondary reagents conjugated to DyLightŪ 488 or DyLightŪ 594 for detection of primary antibodies using green (493Ex/518Em) or red (593Ex/618Em) filter sets.
 
Catalog #
Secondary Antibody Description
Host
Applications
Size
Datasheet
MS3011 Goat Anti-Mouse Ig:DyLigthtŪ 488 Goat pAb ICC,IHC 500µl
MS3031 Goat Anti-Mouse Ig:DyLigthtŪ 594 Goat pAb ICC,IHC 500µl
RS3261 Goat Anti-Rabbit Ig:DyLigthtŪ 488 Goat pAb ICC,IHC 500µl
RS3271 Goat Anti-Rabbit Ig:DyLigthtŪ 594 Goat pAb ICC,IHC 500µl

Applications: ICC = immunocytochemistry, IHC = immunohistochemistry
Reactivity: H = Human, R = Rat, M = Mouse, C = Chicken, F = Frog

 

 
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